Development and evaluation of a monoclonal antibody-based competitive ELISA for the detection of antibodies against H7 avian influenza virus
نویسندگان
چکیده
Abstract Background H7 subtype avian influenza has caused great concern in the global poultry industry and public health. The conventional serological subtype-specific diagnostics is implemented by hemagglutination inhibition (HI) assay despite lengthy operation time. In this study, an efficient, rapid high-throughput competitive enzyme-linked immunosorbent (cELISA) was developed for detection of antibodies against virus (AIV) based on a novel monoclonal antibody specific to hemagglutinin (HA) protein AIV. Results reaction parameters including antigen coating concentration, concentration serum dilution ratio were optimized detection. specificity cELISA tested using antisera H1 ~ H9, H11 H14 AIVs other viruses. selected cut-off values rates chicken, duck peacock sera 30.11, 26.85 45.66% receiver-operating characteristic (ROC) curve analysis, respectively. With HI test as reference method, minimum limits positive reached 2 0 , 1 ? titer, Compared test, diagnostic accuracy 100, 98.6, 99.3% testing total 400 clinical samples, Conclusions summary, study provided reliable, specific, sensitive species-independent technique antibody, which applicable large-scale surveillance vaccination efficacy evaluation programs.
منابع مشابه
Development of blocking ELISA for detection of antibodies against avian influenza virus of the H7 subtype.
BACKGROUND AND PURPOSE The conventional method used for subtyping of antibodies against avian influenza viruses is hemagglutination inhibition (HI) test. However, the HI test is laborious and requires preparation of antigen from viable viruses that might be hazardous. The aim of this study was to develop a blocking enzyme-linked immunosorbent assay (B-ELISA) for detection of antibody of avian i...
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ژورنال
عنوان ژورنال: BMC Veterinary Research
سال: 2021
ISSN: ['1746-6148']
DOI: https://doi.org/10.1186/s12917-021-02772-6